Efficacy of testicular sperm chromatin condensation assay using aniline blue-eosin staining in the IVF-ET cycle
نویسندگان
چکیده
منابع مشابه
Efficacy of testicular sperm chromatin condensation assay using aniline blue-eosin staining in the IVF-ET cycle
OBJECTIVE This study was performed to evaluate testicular sperm chromatin condensation using aniline blue-eosin (AB-E) staining and its effects on IVF-ET. METHODS Chromatin condensation was analyzed using AB-E staining in 27 cases of testicular sperm extraction. There were 19 cases of obstructive azoospermia (OA) and 8 cases of non-obstructive azoospermia (NOA) in IVF-ET. Mature sperm heads w...
متن کاملEvaluation of Protamine Level in Human Sperm Samples Using Chromomycin A3 and Aniline Blue Staining
Background: Current microscopic experimental methods cannot diagnose DNA damages present in spermatozoa .Therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. As reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. Also, chromomycin A3 is used for evaluation of the ...
متن کاملThe utility of sperm DNA damage assay using toluidine blue and aniline blue staining in routine semen analysis
OBJECTIVE The aim of the present study was to examine the relationship among male age, strict morphology, and sperm chromatin structure and condensation. METHODS Sperm samples from a total of 100 men underwent semen analysis, and sperm chromatin structure and condensation were assessed with toluidine blue (TB) and aniline blue (AB) tests. RESULTS Prevalence of strict morphology of less than...
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The main purpose of this study was to determine the possible relationship between chromatin condensation (Aniline Blue staining), the morphology of spermatozoa according to strict criteria, and the fertilization, cleavage and pregnancy rate in an intracytoplasmic sperm injection (ICSI) programme. A total of 60 patients were divided into two groups (27 versus 34) according to sperm stainability ...
متن کاملevaluation of protamine level in human sperm samples using chromomycin a3 and aniline blue staining
background: current microscopic experimental methods cannot diagnose dna damages present in spermatozoa .therefore, some methods are needed to address the abnormality of the genetic material status on the sperm samples. as reported by many investigators aniline blue staining technique has been used for identifying sperm chromatin condensation. also, chromomycin a3 is used for evaluation of the ...
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ژورنال
عنوان ژورنال: Clinical and Experimental Reproductive Medicine
سال: 2011
ISSN: 2233-8233
DOI: 10.5653/cerm.2011.38.3.142